| 產(chǎn)品編號 | bs-4128R-Bio |
| 英文名稱 | Rabbit Anti-MAP4/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的微管相關(guān)蛋白4抗體 |
| 別 名 | DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 細胞周期蛋白 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 121kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAP4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The MAP4 (Microtubule associated protein 4)is a non neuronal microtubule associated protein. It promotes microtubule assembly.The phosphorylation of the Pro rich region in the C-terminus negatively regulates MAP4 activity to promote microtubule assembly. Function: Non-neuronal microtubule-associated protein. Promotes microtubule assembly. Subunit: Interacts with SEPT2; this interaction impedes tubulin-binding. Subcellular Location: Cytoplasm, cytoskeleton. Post-translational modifications: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338. Similarity: Contains 4 Tau/MAP repeats. Database links: Entrez Gene: 4134 Human Entrez Gene: 17758 Mouse Omim: 157132 Human SwissProt: P27816 Human SwissProt: P27546 Mouse Unigene: 517949 Human Unigene: 217318 Mouse Unigene: 443428 Mouse Unigene: 484451 Mouse Unigene: 203122 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
