| 產(chǎn)品編號 | bsm-41414M |
| 英文名稱 | SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse mAb |
| 中文名稱 | SARS冠狀病毒2核衣殼蛋白單克隆抗體 |
| 別 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Prot |
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Specific References (5) | bsm-41414M has been referenced in 5 publications.
[IF=7.344] Lassaunière, Ria. et al. Preclinical evaluation of a candidate naked plasmid DNA vaccine against SARS-CoV-2. Npj Vaccines. 2021 Dec;6(1):1-13 ELISA ; Mouse.
[IF=5.897] Larry L. Luchsinger. et al. Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients. J Clin Microbiol. 2020 Nov;58(12):e02005-20 ELISA ; Human.
[IF=3.752] Anders Frische. et al. Optimization and evaluation of a live virus SARS-CoV-2 neutralization assay. PLOS ONE. 2022 Jul;17(7):e0272298 ELISA ; Monkey.
[IF=3.09] Nesbitt, Daniel J.. et al. Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats. Bmc Infect Dis. 2021 Dec;21(1):1-9 ELISA ; Human.
[IF=2.74] Daniel K. Jin. et al. Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and “Reopening”. Plos One. 2021 Apr;16(4):e0250319 ELISA ;
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| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 7E1B |
| 交叉反應 | SARS-CoV-2 |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | ≥1 mg/ml |
| 免 疫 原 | Recombinant SARS-CoV-2 Nucleocapsid Protei: full length |
| 亞 型 | IgG2b |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng
Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng
Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng
Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng
Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng
Lane 6:SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng
Primary: Anti- SARS-CoV-2 N protein (bs-41414M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
