| 產(chǎn)品編號(hào) | bsm-60328R |
| 英文名稱 | SIGLEC1 Recombinant Rabbit mAb |
| 中文名稱 | CD169重組兔單抗 |
| 別 名 | CD 169; CD-169; dJ1009E24.1; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; Sialic acid binding Ig like lectin 1; Sialic acid binding immunoglobulin like lectin 1; Siglec 1; SN; sialoadhesin; SN_HUMAN. |
| 研究領(lǐng)域 | 免疫學(xué) 微生物學(xué) b-淋巴細(xì)胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | B4H12 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:200-500,IHC-F=1:200-500,IF=1:50,Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 186 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SIGLEC1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]. Function: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Subunit: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Subcellular Location: Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 16 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9BZZ2 Gene ID: 6614 Database links: Entrez Gene: 6614 Human Entrez Gene: 20612 Mouse Omim: 600751 Human SwissProt: Q9BZZ2 Human SwissProt: Q62230 Mouse Unigene: 31869 Human Unigene: 1374 Mouse CD169，又稱sialoadhesin（Sn）或Siglee-1，這是一種200kDa的I型跨膜糖蛋白，由炎性巨噬細(xì)胞特異性表達(dá)，在細(xì)胞-細(xì)胞或細(xì)胞-基質(zhì)的相互作用中發(fā)揮主要作用?？勺R(shí)別乳癌細(xì)胞的MUC1（CD227）和T細(xì)胞的CD43。CD169在脾、淋巴結(jié)、骨髓的基質(zhì)巨噬細(xì)胞上表達(dá)最強(qiáng)，在肝、腸、肺的長(zhǎng)駐巨噬細(xì)胞上中等表達(dá)，慢性炎組織的巨噬細(xì)胞，例如類風(fēng)濕性滑膜、動(dòng)脈粥樣硬化性斑塊，也強(qiáng)烈表達(dá)CD169。 |
| 產(chǎn)品圖片 |
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary incubation condition: 1 hour at room tem
Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room
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