| 產(chǎn)品編號 | bsm-51604M |
| 英文名稱 | PPP2R1B Mouse mAb |
| 中文名稱 | 蛋白質(zhì)磷酸酶2調(diào)節(jié)亞基1B單克隆抗體 |
| 別 名 | 2AAB_HUMAN; beta isoform of regulatory subunit A, protein phosphatase 2; PP2A A beta; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A, subunit A, PR65 beta isoform; PP2A, subunit A, R1 beta isoform; PPP2R1B; PR65B; protein phosphatase 2(formerly 2A), regulatory subunit A(PR 65), beta isoform; protein phosphatase 2(formerly 2A), regulatory subunit A, beta isoform; Protein phosphatase 2 regulatory subunit A alpha isoform; protein phosphatase 2, structural/regulatory subunit A, beta isoform; Serine threonine protein phosphatase 65 kDa; serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 激酶和磷酸酶 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | H6T2 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:25,IHC-F=1:25,IF=1:25,Flow-Cyt=1:25,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PPP2R1B. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Function: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Subunit: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with SGOL1. Interacts with RAF1. Similarity: Belongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats. SWISS: P30154 Gene ID: 5519 Database links: Entrez Gene: 5519 Human SwissProt: P30154 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Jurkat cell lysates
Lane 2: Human brain tissue lysates
Lane 3: Mouse brain tissue lysates
Lane 4: Mouse lung tissue lysates
Lane 5: Rat brain tissue lysates
Primary: Anti-PPP2R1B (bsm-51604M) at 1/4000 dilution
Secondary: IRDye800CW
Paraformaldehyde-fixed, paraffin embedded (Human spleen sections ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C f
U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PPP2R1B) monoclonal Antibody, Unconjugated (bsm-51604M) 1:25, 90 minutes at 37°C; followe
Blank control:Jurkat.
Primary Antibody (green line): Mouse Anti-PPP2R1B antibody (bsm-51604M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:400
Protocol
The cells were fixed
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