| 產(chǎn)品編號(hào) | bs-2962R |
| 英文名稱(chēng) | Syncytin 1 Rabbit pAb |
| 中文名稱(chēng) | 合胞素1抗體 |
| 別 名 | HERV-W_7q21.2 provirus ancestral Env polyprotein; Endogenous retrovirus group W member 1; env; Env-W; Envelope polyprotein gPr73; Enverin; ENW1_HUMAN; ERVW; ERVW-1; Gp24; Gp50; HERV-7q Envelope protein; HERV-W envelope protein; HERVW; SU; Syncytin 1; Syncytin; Syncytin-1; TM; Transmembrane protein; Surface protein. |
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Specific References (5) | bs-2962R has been referenced in 5 publications.
[IF=17.694] Whitlock, Jarred M.. et al. Cell surface-bound La protein regulates the cell fusion stage of osteoclastogenesis. NAT COMMUN. 2023 Feb;14(1):1-19 WB ; Mouse,Human.
[IF=5.168] Díaz-Carballo et al. Cytotoxic stress induces transfer of mitochondria-associated human endogenous retroviral RNA and proteins between cancer cells. (2017) Oncotarget. 8:95945-95964 other ; Human.
[IF=4.757] Rachana Pandit. et al. Canine Coronavirus Infection Modulates the Biogenesis and Composition of Cell-Derived Extracellular Vesicles. BIOMEDICINES. 2023 Mar;11(3):976 Dot Blot ; Cat.
[IF=4.43] Díaz-Carballo, David, et al. "Therapeutic potential of antiviral drugs targeting chemorefractory colorectal adenocarcinoma cells overexpressing endogenous retroviral elements." Journal of Experimental & Clinical Cancer Research 34.1 (2015): 81. WB ; Human.
[IF=4.025] Li Zhuo-Hang. et al. The roles of ADAMDEC1 in trophoblast differentiation during normal pregnancy and preeclampsia. MOLECULAR HUMAN REPRODUCTION. 2022 May;: WB ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 微生物學(xué) 細(xì)菌及病毒 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 59 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Syncytin 1: 451-538/538 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. SU mediates receptor recognition. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide (By similarity). Seems to recognize the type D mammalian retrovirus receptors SLC1A4 and SLC1A5, as it induces fusion of cells expressing these receptors in vitro. The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes. Function: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. SU mediates receptor recognition. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide (By similarity). Seems to recognize the type D mammalian retrovirus receptors SLC1A4 and SLC1A5, as it induces fusion of cells expressing these receptors in vitro. The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes (By similarity). Subunit: The mature envelope protein (Env) consists of a trimer of SU-TM heterodimers attached probably by a labile interchain disulfide bond. Interacts with the C-type lectin CD209/DC-SIGN. Subcellular Location: Transmembrane protein: Cell membrane; Single-pass type I membrane protein (By similarity). Surface protein: Cell membrane; Peripheral membrane protein (By similarity). Note=The surface protein is not anchored to the membrane, but localizes to the extracellular surface through its binding to TM (By similarity). HERV-W_7q21.2 provirus ancestral Env polyprotein: Virion (By similarity). Tissue Specificity: Expressed at higher level in placental syncytiotrophoblast. Expressed at intermediate level in testis. Seems also to be found at low level in adrenal tissue, bone marrow, breast, colon, kidney, ovary, prostate, skin, spleen, thymus, thyroid, brain and trachea. Both mRNA and protein levels are significantly increased in the brain of individuals with multiple sclerosis, particularly in astrocytes and microglia. Post-translational modifications: Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as a inactive precursor that is heavily N-glycosylated and processed likely by furin in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence [KR]-X-[KR]-R. The intracytoplasmic tail cleavage by the viral protease that is required for the fusiogenic activity of some retroviruses envelope proteins seems to have been lost during evolution. The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion (By similarity). Similarity: Belongs to the gamma type-C retroviral envelope protein family. HERV class-I W env subfamily. SWISS: Q9UQF0 Gene ID: 30816 Database links: Entrez Gene: 2086 Human Entrez Gene: 30816 Human Entrez Gene: 405754 Human Omim: 604659 Human SwissProt: O42043 Human SwissProt: O71037 Human SwissProt: P10267 Human SwissProt: P60507 Human SwissProt: P60508 Human SwissProt: P61550 Human SwissProt: P61565 Human SwissProt: P61566 Human SwissProt: P61567 Human SwissProt: P61570 Human SwissProt: Q14264 Human SwissProt: Q69384 Human SwissProt: Q902F8 Human SwissProt: Q902F9 Human SwissProt: Q96L62 Human SwissProt: Q9N2J8 Human SwissProt: Q9N2J9 Human SwissProt: Q9N2K0 Human SwissProt: Q9NX77 Human SwissProt: Q9UKH3 Human SwissProt: Q9UQF0 Human Unigene: 250693 Human Unigene: 631996 Human 合胞素(Syncytin)是一類(lèi)由人俘獲的逆轉(zhuǎn)錄病毒囊膜蛋白,與胎盤(pán)的形態(tài)發(fā)生中細(xì)胞滋養(yǎng)層到合胞滋養(yǎng)層的分化過(guò)程相關(guān)。Syncytin與人免疫缺陷病毒I型(HIV-1)囊膜蛋白(Env)在結(jié)構(gòu)上具有相似的特點(diǎn),二者可能具有相似的膜融合機(jī)制。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: JEG-3 (Human) Cell Lysate at 30 ug
Lane 2: JAR (Human) Cell Lysate at 30 ug
Lane 3: U2os (Human) Cell Lysate at 30 ug
Lane 4: U251 (Human) Cell Lysate at 30 ug
Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 6: HT1080 (Human) Cell Lysat
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-Syncytin 1 (bs-2962R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/58 kD
Observed band size: 58kD
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