| 產(chǎn)品編號(hào) | bs-1550R |
| 英文名稱 | NCX1 Rabbit pAb |
| 中文名稱 | 鈉鈣交換蛋白1抗體 |
| 別 名 | Na+/Ca2+exchanger 1; CNC; DKFZp779F0871; MGC119581; FLJ37694; FLJ43417; Na+/Ca2+exchange protein 1; Na+/Ca2+exchanger; NCX 1; NCX; SLC8A1; SLC8A1 protein; Sodium Calcium Exchanger; Sodium/calcium exchanger 1; Solute carrier family 8 member 1; NAC1_HUMAN. |
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Specific References (2) | bs-1550R has been referenced in 2 publications.
[IF=3.201] Zhang J et al. TRPV4 Complexes With the Na/Ca 2 Exchanger and IP 3 Receptor 1 to Regulate Local Intracellular Calcium and Tracheal Tension in Mice. Front. Physiol., 06 December 2019. Co-IP&WB ; Mouse.
[IF=1.922] Zhu Y et al. MicroRNA?34a mediates atrial fibrillation through regulation of Ankyrin?B expression.Mol Med Rep. 2018 Jun;17(6):8457-8465. WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 轉(zhuǎn)運(yùn)蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat,GuineaPig (predicted: Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 106 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NCX1: 801-900/971 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma(plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM]. Subcellular Location: Cell membrane. Tissue Specificity: Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung. Similarity: Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. Contains 2 Calx-beta domains. SWISS: P32418 Gene ID: 6546 Database links: Entrez Gene: 6546 Human Entrez Gene: 20541 Mouse Entrez Gene: 100328570 Rabbit Omim: 182305 Human SwissProt: P48766 Guinea pig SwissProt: P32418 Human SwissProt: P70414 Mouse Unigene: 31961 Human Unigene: 468274 Human Unigene: 728911 Human Unigene: 265834 Mouse Unigene: 118972 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: U251 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-NCX1 (bs-1550R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicte
Sample:
HL-60(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-NCX1 (bs-1550R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kD
Observed band size: 118 kD
Sample:
293T(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-NCX1 (bs-1550R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kD
Obser
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30mi
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Antibody(bs-1550R) at
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-NCX1 antibody (bs-1550R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
Th
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