| 產(chǎn)品編號(hào) | bs-0596R |
| 英文名稱 | Dopamine beta Hydroxylase Rabbit pAb |
| 中文名稱 | 多巴胺β羥化酶抗體 |
| 別 名 | DBH; DBM; Dopamine beta hydroxylase; Dopamine-beta-Hydroxylase; Dopamine beta monooxygenase; DOPO_RAT; DOPO_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DBH: 471-550/621 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Dopamine-beta-Hydroxylase (DBH), Dopamine can be transformed into Noradrenalin. Conversion of dopamine to noradrenaline. [SUBUNIT] Homotetramer composed of two non-covalently bound disulfide-linked dimers. [SUBCELLULAR LOCATION] Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (Potential). Belongs to the copper type II ascorbate-dependent monooxygenase family. Function: Conversion of dopamine to noradrenaline. Subunit: Homotetramer composed of two non-covalently bound disulfide-linked dimers (By similarity). Subcellular Location: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen (By similarity). Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (By similarity). Tissue Specificity: Chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system. Similarity: Belongs to the copper type II ascorbate-dependent monooxygenase family. Contains 1 DOMON domain. SWISS: P09172 Gene ID: 1621 Database links: Entrez Gene: 1621 Human Entrez Gene: 13166 Mouse Omim: 223360 Human Omim: 609312 Human SwissProt: P09172 Human SwissProt: Q64237 Mouse Unigene: 223858 Human Unigene: 591890 Human Unigene: 167781 Mouse Unigene: 87166 Rat Dopamine-β-Hydroxylase（DBH）—多巴胺β羥化酶。可以將多巴胺轉(zhuǎn)化成去甲腎上腺素。是腎上腺素能神經(jīng)元的標(biāo)志。 |
| 產(chǎn)品圖片 |
Sample:
Jurkat (Human) Cell Lysate at 40 ug
Primary: Anti-Dopamine beta Hydroxylase (bs-0596R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Anti
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Dopamine beta Hydroxylase antibody (bs-0596R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（oran
|
