| 產(chǎn)品編號(hào) | bs-0450R |
| 英文名稱 | Annexin V Rabbit pAb |
| 中文名稱 | 膜粘連蛋白5抗體 |
| 別 名 | Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A5; ANX5; ANXA5; Calphobindin I; CBP I; CBP-I; Endonexin II; ENX 2; ENX2; Lipocortin V; PAP I; Placental anticoagulant protein I; PAP-I; Placental anticoagulant protein 4; PP 4; PP4; Thromboplastin inhibitor; VAC alpha; Vascular anticoagulant alpha; Vascular anticoagulant-alpha; ANXA5_HUMAN; Anchorin CII; Annexin-5; VAC-alpha. |
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Specific References (2) | bs-0450R has been referenced in 2 publications.
[IF=15.84] Yulian Wang. et al. Delivering Antisense Oligonucleotides across the Blood‐Brain Barrier by Tumor Cell‐Derived Small Apoptotic Bodies. 2021 May 04 FC ; Mouse.
[IF=9.988] Jing-zhi Li. et al. RNA binding protein YTHDF1 mediates bisphenol S-induced Leydig cell damage by regulating the mitochondrial pathway of BCL2 and the expression of CDK2-CyclinE1. ENVIRON POLLUT. 2023 Jan;:121144 FCM ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞凋亡 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | recombind human Annexin V |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]. Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Subunit: Interacts with PDCD6. Post-translational modifications: S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. DISEASE: Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P08758 Gene ID: 308 Database links: Entrez Gene: 308 Human Entrez Gene: 11747 Mouse Omim: 131230 Human SwissProt: P08758 Human SwissProt: P48036 Mouse Unigene: 480653 Human Unigene: 1620 Mouse Unigene: 3318 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Mouse Uterus tissue lysates
Lane 4: Mouse Spleen tissue lysates
Lane 5: Mouse Stomach tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Rat Placenta tissue
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Annexin V? (bs-0450R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35 kD
Sample:
PANC-1(Human) Cell Lysate at 30 ug
Primary: Anti-Annexin V? (bs-0450R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35 kD
Sample: Lung (Mouse) Lysate at 40 ug
Primary: Anti- Annexin V (bs-0450R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat ser
Tissue/cell: human Neurological glioblastoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat s
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goa
Blank control: RSC96 (blue).
Primary Antibody:Rabbit Anti-Annexin V antibody(bs-0450R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-ra
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